The mathematical principle of natural science refers to the application and function of mathematics in natural science, which is the basis for the establishment of natural science theory. Mathematical principles not only provide tools for describing, analyzing and predicting nature, but also help scientists discover laws, explain phenomena and deduce theories. In natural science, mathematics is often used in physics, chemistry, biology and other fields to provide mathematical models and calculation methods for scientific research. Mathematical model is one of the most commonly used tools in natural science. By establishing mathematical models, scientists can abstract complex natural phenomena into simple mathematical relationships, so as to better understand and study these phenomena. Calculation method is another important mathematical principle in natural science. In many practical problems, because of the complexity of the problem or the direct experimental observation, it is necessary to get the answer by means of calculation.