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Biotechnology course
Brief introduction of courses in Beijing Agricultural College
I. Department of Biotechnology
Introduction to compulsory courses
Course Name: General Biology
Course content: introduce the origin of organisms, species classification, biological community, population ecology, organizational structure, organ development, reproductive growth process, biological variation and evolution law, genetic basis of life alternation, and molecular biology principles of biodiversity.
Textbook: General Biology, edited by Gu Dexing, Higher Education Press.
Course Name: Biochemistry
Course content: This course systematically introduces the basic principles of biochemistry. The main course consists of three parts. 1. Structure and function of life macromolecules, mainly introducing the structure and function of protein, nucleic acid, carbohydrate and lipid. Secondly, it is the metabolic pathway of main substances and energy in life activities, and introduces the molecular mechanism of biosynthesis, biodegradation and biological oxidation of main life macromolecules. Third, the storage, expression and transmission of life information. This paper mainly introduces the basic principles of DNA transcription and protein synthesis.
Prerequisite: organic chemistry
Textbook: Principles of Biochemistry of Leininger, 2000 (original edition).
Course Name: Plant Physiology
Course content: Plant physiology is a science to study the law of plant life activities, and it is a compulsory basic course for planting students. According to the system of "Metabolic Physiology-Developmental Physiology-Stress Physiology", this course introduces water metabolism of plants, mineral nutrition of plants, photosynthesis of plants, respiration of plants, transformation and transportation of organic matter in plants, cell signal transduction in plants, plant growth substances, photomorphogenesis, growth physiology of plants, reproductive physiology of plants, maturity and senescence physiology of plants, and resistance physiology of plants. On the basis of inheriting and developing the traditional contents of plant physiology, this course pays attention to the combination of theory and production practice, introduces the relevant contents of molecular biology, absorbs the latest research results at home and abroad in recent years, and provides a theoretical basis for students majoring in planting.
Subject: Biotechnology, Forestry and Plant Production.
Prerequisites: Botany and Biochemistry
Textbook: Plant Physiology, edited by Zhong Wang, China Agricultural Publishing House.
Plant Physiology, edited by Pan Ruichi and Dong Yude, Higher Education Press.
Course Name: Animal Physiology Ⅰ
Course content: Animal physiology is a science that studies the laws of normal life activities of animals, and studies the normal functional activities and regulation of blood, circulation, respiration, digestion, urinary system, nervous system and endocrine system of animals. On the whole, animal physiology should not only clarify the relationship between the functions of each organ system and the functional activities of each part, but also clarify the changing law of the activities of each organ system when animals interact with the environment, and explore their activities at the cellular and molecular levels.
Prerequisite courses: general biology, biochemistry
Textbook: Animal Physiology, edited by Li,,, and China Agricultural Science and Technology Press.
Course Name: General Genetics Ⅰ
Course content: Genetics is a science that studies the laws of biological inheritance and variation, and it is a professional basic course for biotechnology majors. Genetics takes genes as the center and revolves around the changing law of biological characters, so genetics is the science of studying genes. Genetics is the basic discipline of life science, and its development includes two stages: classical genetics and modern genetics. The research field can be divided into four main branches: traditional genetics, cytogenetics, molecular genetics and biogenetics. The research contents include the cytological basis of inheritance, Mendelian inheritance law, chromosome structural variation and number variation, genetic analysis of bacteria and viruses, quantitative traits and polygene inheritance, molecular basis of inheritance and gene recombination, gene mutation, cytoplasmic inheritance, population inheritance and so on. Through the study of this course, students can fully grasp the basic knowledge and research methods of genetics, have an understanding of complex life phenomena in line with objective laws, understand the new achievements of modern genetics development, and combine them with traditional technologies in agriculture, medicine, industry and other fields to lay a foundation for further study of biotechnology, genetic engineering and related branches of genetics.
Course Name: Cell Biology
English name: cell biology
Academic performance: 2.5 Total class hours: 45 (Theory 36, Experiment 9)
Course content: Cell biology is a science that studies the basic life activities of cells and is one of the basic disciplines of modern life science. This course mainly takes cells as the basic unit, and introduces the ultrastructure and function of cells at micro, submicroscopic and molecular levels, including the basic characteristics of cells, cell-free life-virus and its relationship with cells, and the concepts and characteristics of prokaryotic cells, eukaryotic cells, mycoplasma and other cells. The specialized structure of cytoskeleton and cell surface and the connection mode of cell connection; Intercellular communication and signal transmission; The structure and function of cytoplasmic matrix and intima system, the secretion of intracellular protein and the assembly of cell structure; Concept, structure and function of cytoskeleton; Regulation mechanism of cell proliferation process; Cell differentiation and gene expression regulation; Structural changes of cell aging, concept, morphological and biochemical characteristics and molecular mechanism of apoptosis. At the same time, the basic methods, research progress and main development direction of cell biology are briefly introduced.
Subject: Biotechnology major.
Basic chemistry, organic chemistry, analytical chemistry, botany, basic biochemistry, plant physiology, genetics.
Textbook: Cell Biology, edited by Zhai Zhonghe, Higher Education Press, 2002.
Cell Biology, edited by Zheng Guo, Higher Education Press, 1992 (reference book).
Course Name: Biochemical Experimental Technology
Course content: Biochemical experiment is one of the most basic and important technologies for studying biological macromolecules, and it is an indispensable part in the development of life science. This course is a comprehensive and systematic comprehensive training on the basis of mastering some basic biochemical theories, basic operations and professional knowledge. Pay attention to cultivating students' ability to analyze and solve problems independently, and constantly improve their experimental skills. Biochemical experiments mainly focus on protein's chemistry, enzymology and immunology. Select representative experimental contents, and integrate spectrum, chromatography, electrophoresis, protein sequencing, immunology and other technologies according to the analysis target. In the aspect of experimental technology, we should not only consider the mutual connection and integrity of contents and methods, but also consider that the teaching of each unit has a relatively independent focus. Main experimental contents: protein preparation technology (focusing on chromatography and determination of enzyme activity), protein detection technology (determination of molecular weight and isoelectric point of protein) and immunology technology (antibody titer detection technology).
Subject: Biotechnology major.
Basic biochemistry, organic chemistry, general chemistry and general physics.
Teaching material: to be determined
Course Name: Experimental Statistical Analysis
Course content: learn and master the basic knowledge of biological experiments, understand the design of experimental schemes and the basic methods of controlling experimental errors in the process of biological experiments. Learn the basic skills of investigating, summarizing and analyzing test data to obtain reliable test results. Learn the basic principles and procedures of statistical inference and make scientific conclusions about the results of scientific experiments. This course is an essential professional basic course in biology, which is related to whether scientific experiments can be carried out correctly and is a necessary means to obtain correct conclusions.
Subject: Biotechnology major.
Prerequisite courses: Mathematics, Probability, Basic Biology.
Textbook: self-edited
Course number: 0 1 1009
Course Name: Principles of Genetic Engineering
Principles of genetic engineering
Academic performance: 5 total hours: 80 (theory 50, experiment 30)
Course content: Genetic engineering is the operation process of introducing foreign genes into recipient cells through in vitro recombination, so that the genes can be replicated, transcribed, translated and expressed in recipient cells, also known as recombinant DNA technology. Genetic engineering is a comprehensive biotechnology discipline, and gene manipulation provides a powerful means to explore the mysteries of life. In recent years, recombinant DNA technology has made great progress in basic theoretical research and practical application. The principle of genetic engineering introduces the upstream technology of genetic engineering based on molecular biology and molecular genetics. Genetic engineering mainly includes the enzymatic basis of gene cloning, molecular cloning vectors, isolation and identification of target genes, construction of gene library, expression and regulation of cloned genes, main technical principles of gene manipulation, application and development of plant genetic engineering and recombinant DNA technology. Using genetic engineering methods, we can create new gene combinations according to people's pre-designed ways, actively transform the genetic characteristics of organisms, and effectively promote the development of industry, agriculture and medicine.
Prerequisite courses: microbiology, biochemistry, genetics, molecular biology,
Teaching material: to be determined
Course Name: Plant Tissue Culture and Cell Engineering
Course content: the concepts of plant tissue culture and cell engineering, the principle of plant tissue culture, the disinfection of plant materials and utensils, the preparation of culture medium, the technology of plant cell fusion, the principle and method of cell mutant screening, and the application of plant tissue culture and cell engineering technology in agriculture.
Subject: Biotechnology major.
Prerequisite courses: genetics, biochemistry, plant physiology
Teaching material: to be determined
Course Name: Molecular Biology
Course content: Molecular biology is a science that studies the function, morphological structure, importance and regularity of biological macromolecules such as nucleic acids. This course focuses on the replication and transmission of genetic information, the structure and function of genes, gene mutation and recombination, the mechanism of gene regulation and expression, and the basic principles of gene operation and application. The main teaching contents include: the characteristics of genetic material, the characteristics of genes, the transmission process of genetic information, the expression and regulation of prokaryotic genes, the expression and regulation of eukaryotic genes and molecular biology research techniques.
Subject: Biotechnology major.
Prerequisite courses: general biology, genetics
Textbook: Molecular Biology, edited by Yan Longfei and Ston Zhang, China Agricultural University Press.
Course Name: Molecular Biology Experiment
Course content: This course mainly introduces the separation and purification of plasmid DNA, the application of restriction endonuclease, polyacrylamide gel electrophoresis (PAGE), polymerase chain reaction (PCR), Soutern hybridization and Western hybridization.
Subject: Biotechnology major.
Prerequisite: molecular biology
Teaching material: to be determined
Course Name: Animal Embryo Engineering
Course content: Animal embryo engineering mainly teaches the basic parts of embryo engineering, including gamete production, fertilization and early embryo development, as well as the principles and methods of embryo culture, in vitro fertilization, embryo transfer, embryo preservation, embryo separation, sex determination principle and control, embryo nuclear transfer, somatic cell cloning, chimera, animal embryonic stem cells, transgenic animals and so on.
Subject: Biotechnology major.
Prerequisite courses: animal reproductive physiology, animal physiology
Textbook: self-edited
Course Name: General Genetics Ⅱ
Course content: Genetics is a science that studies biological heredity, variation and its laws, and it is an important professional basic course for all majors of plant science. Through the study of this course, students should fully grasp the basic knowledge and general research methods of genetics, deeply understand the basic laws and mechanisms of heredity and variation, and understand the new progress of modern genetics, so as to lay the foundation for further study of branches of genetics and subsequent related courses. The contents of this course mainly include: the cytological basis of heredity, the three basic laws of heredity and their research methods, the variation of chromosome structure and number, the inheritance of bacteria and viruses, the molecular basis of genetic material and its gene mutation, cytoplasmic inheritance, the inheritance of quantitative traits, inheritance and evolution, etc.
Elective subjects: major in plant production and forestry.
Prerequisite courses: organic chemistry, biochemistry, biostatistics
Textbook: Genetics, edited by Liu Zudong, Higher Education Press.
General Genetics, edited by Song Yunchun, Wuhan University Press.
Course Name: Animal Biochemistry Ⅱ
Course content: Animal biochemistry is a professional basic course for veterinary specialty. Through the study of this topic, we can understand the contents of biochemical research, the concepts of chemical composition and metabolism of animal body, the chemical composition, structure and physicochemical properties of protein, the chemical composition and structural characteristics of nucleic acid, the concept, structure and function of enzyme and its action principle, the relationship between vitamins and coenzymes, and the physiological function and deficiency of vitamins. Master the concepts of sugar metabolism, energy supply and gluconeogenesis, biological oxidation, two main respiratory chains, lipid metabolism in vivo, protein, the relationship between sugar and lipid metabolism, the process of nucleic acid metabolism and protein biosynthesis, the adjustment of acid-base balance, the metabolism of water, sodium, potassium, calcium and phosphorus in vivo and the biochemistry of some tissues and organs.