A, the chemical bond limiting the action of enzyme is phosphodiester bond, so A is correct;

B, as can be seen from the figure, the sticky ends produced by enzyme A and enzyme B are the same and can be connected with each other, so B is wrong;

C and A enzymes can cut the original DNA into four segments, that is, there are three incisions on the DNA molecule, that is, the A enzyme has three recognition sequences; B-enzyme cuts the DNA with the size of 2 100 into two fragments with the size of 1900 and 200 respectively, and then cuts the DNA with the size of 1400 into two fragments with the size of 800 and 600 respectively, which shows that B-enzyme has two incisions on the DNA molecule, that is, B-enzyme has two recognition sequences, so C is correct.

D and B enzymes have at least two recognition sequences, and at least three kinds of DNA fragments can be obtained by cutting DNA molecules with B enzyme only, so D is correct.

So choose B.