The first chapter is introduction.
Section 1 Nature, Content and Classification of Biological Separation Engineering
First, the essence of biological separation engineering
Second, the research content of biological separation engineering
Three, the classification of biological separation process
In the second quarter, the general process of biological separation engineering
1. Pretreatment of fermentation broth
Second, product extraction
Third, the refinement of products.
Fourth, finished product processing.
Five, biological separation and purification process selection basis
Section III Characteristics of Biological Separation Process
Firstly, the system of biological separation process is special.
Second, the process of biological separation is special.
Thirdly, the cost of biological separation process is special.
The fourth quarter development trend of biological separation engineering
First, the development trend of biological separation engineering
Second, the problems that should be paid attention to in the research of biological separation engineering
Think about a problem
Chapter II Pretreatment of Fermentation Broth
Section 1 Pretreatment Method of Fermentation Broth
First, the general characteristics of fermentation broth
Second, the purpose and requirements of fermentation broth pretreatment
Third, the pretreatment method of fermentation broth
Part 2: filtration of fermentation broth,
First, the purpose of fermentation broth filtration
Second, the influencing factors of fermentation broth filtration
Third, the method of fermentation broth filtration
Fourthly, methods to improve filtration performance.
Verb (abbreviation of verb) Selection of filter media
6. Optimization of filtration operation conditions
Seven, filtration equipment
Think about a problem
Chapter III Cell Separation Technology
Section 1 Cell separation
First, filter
Secondly, centrifugal precipitation.
Cell division in the second quarter
First, the structure of the cell wall
Second, the dynamics of cell division.
Third, the method of cell fragmentation.
Section III Dissolution and Renaturation of Intracellular Products
I. Inclusion bodies and their formation
Second, the separation and dissolution of inclusion bodies.
Thirdly, protein renaturation.
Think about a problem
Chapter IV Precipitation Technology
Section 1 Overview
Section 2 Surface Characteristics of protein
I. Hydrophilicity and Hydrophobicity of protein Surface
Second, the charge on the surface of protein.
Thirdly, the stability of protein colloid.
Section 3 protein precipitation method
I. salting-out method
Second, the organic solvent precipitation method
Third, the isoelectric point precipitation method
IV. Non-ionic polymer precipitation method
Verb (abbreviation of verb) denatures and precipitates.
Six, the formation of salt complex precipitation
Seven. affinity precipitation
Eight. SIS polymer and affinity precipitation
The fourth quarter precipitation technology application
I. protein
Second, polysaccharide.
Thirdly, the study on purification technology of tea saponin.
4. Extraction of chloropropionic acid from Eucommia ulmoides Oliv.
Think about a problem
Chapter V Extraction Technology
Section 1 Basic Concepts
First, the concept, characteristics and classification of extraction
Second, the distribution law.
Three. Distribution coefficient, comparison and separation coefficient
Section II Basic Theory and Process of Liquid-liquid Extraction
First, the basic principle of liquid-liquid extraction
Second, the liquid-liquid extraction type and process calculation
Section III Organic Solvent Extraction
I. extraction and distribution equilibrium of organic solvents
Second, the factors affecting organic solvent extraction
Three. Organic solvent extraction equipment and process
Section IV Aqueous Two-phase Extraction
First, the formation of aqueous two-phase system.
Second, the phase diagram
Thirdly, the distribution equilibrium of aqueous two-phase system.
Four, the main factors affecting the partition coefficient of aqueous two-phase system
Verb (abbreviation of verb) equipment and technology for aqueous two-phase extraction
Section 5 Liquid membrane extraction
I. Liquid film and its classification
Second, the liquid membrane extraction mechanism
Third, the liquid membrane separation operation
Four. Process flow of emulsion liquid membrane separation technology
Potential problems in the process of liquid membrane separation of verb (abbreviation of verb)
Application of liquid membrane separation technology of intransitive verbs
Section 6 Reverse Micellar Extraction
First, micelles and reverse micelles.
Second, reverse micelle extraction.
Thirdly, the preparation of reverse micelles.
Fourthly, the application of reverse micelle extraction.
Section 7 liquid-solid extraction
I. Liquid-solid extraction process
Second, the types of liquid-solid extraction
Third, the influencing factors of leaching
Four, other problems of leaching
Industrial application of verb (verb abbreviation) leaching
Section 8 Supercritical fluid extraction
I. Supercritical fluids
Second, supercritical fluid extraction.
Third, the experimental device and extraction mode of supercritical extraction
4. Selection of supercritical fluid extraction conditions
Five, the basic process of supercritical fluid extraction
6. Application example of supercritical fluid extraction
Section 9 Application and Research Progress of Extraction Technology
Application and research progress of 1. aqueous two-phase extraction technology
Application and research progress of liquid membrane extraction technology
Application and research progress of reverse micelle extraction technology
Application and research progress of supercritical fluid extraction technology
Think about a problem
Chapter VI Membrane Separation Process
Section 1 Overview
First, the concept and characteristics of membrane separation process
Second, the classification of membrane technology
Thirdly, separation membrane.
Pressure-driven membrane process in the second quarter
I. Reverse osmosis and nanofiltration
Second, ultrafiltration and microfiltration.
The third electricity-saving drive electrodialysis-membrane process
First, the basic principle of electrodialysis
Second, electrodialysis transfer process and its influencing factors
Three, electrodialysis membrane
Fourth, application
Section 4 Membrane Contactor-Membrane Extraction
First, the basic principle of membrane extraction
Second, the mass transfer process of membrane extraction
Three. Influencing factors of membrane extraction process
Fourth, application
Section 5 Other membrane separation processes
Firstly, the concentration difference drives the membrane process-pervaporation.
Second, the temperature difference drives the membrane process-membrane distillation.
Section 6 Membrane Separation Process Device
I. Filter cartridge membrane module
Second, the plate-frame membrane module
Three, spiral wound membrane module
Four. Tubular membrane module
Verb (abbreviation of verb) capillary membrane module
Six, hollow fiber membrane module
Think about a problem
Chapter VII Adsorption and Ion Exchange
Section 1 Overview
I. Adsorption process
Second, the characteristics of adsorption and ion exchange
Section 2 Adsorption and Separation of Medium
First, the adsorbent
Second, the ion exchanger
Section III Basic Theory of Adsorption and Ion Exchange
First, the adsorption equilibrium theory
Second, the main factors affecting adsorption
Third, the theory of ion exchange equilibrium.
Section 4 Basic Equipment and Operation
First, the fixed bed adsorption operation
Second, the moving bed adsorber
Third, the expanded bed adsorption operation
Four, fluidized bed adsorption operation
Verb (abbreviation of verb) Calculation and selection of purification efficiency of adsorber
Think about a problem
Chapter VIII Chromatographic Separation Technology
Section 1 Overview of Chromatographic Separation Technology
First, the basic concept of chromatography technology
Second, the classification of chromatography
Third, the operation method of chromatographic system
Section II Adsorption Chromatography
First, the basic principle of adsorption chromatography
Second, adsorption thin-layer chromatography
Thirdly, adsorption column chromatography.
Section III partition chromatography
I. Basic principles
Second, the partition chromatography condition
Third, the basic operation of partition chromatography
Fourth, the application of partition chromatography.
Section 4 ion exchange chromatography
First, the basic principle of ion exchange chromatography technology
Second, the type and structure of ion exchanger
Three. Physical and chemical properties of ion exchanger
Four, the basic operation of ion exchange chromatography
Verb (abbreviation of verb) Application of ion exchange chromatography
Section 5 affinity chromatography
I. Overview of Affinity Chromatography
Second, the principle of affinity chromatography
Three. Affinity chromatographic medium
4. Preparation of affinity chromatography medium
Operation process of affinity chromatography of verb (abbreviation of verb)
Factors affecting affinity chromatography by intransitive verbs
Section 6 Application of Chromatographic Separation Technology
I. Application of Affinity Chromatography
Second, the application of ion exchange chromatography
Third, the application of adsorption chromatography
Fourth, the application of partition chromatography.
Five, the combination of a variety of chromatographic technology application
Think about a problem
Chapter 9 Centrifugal Technology
The first section centrifugal separation principle
First, the principle of centrifugal sedimentation
Second, the principle of centrifugal filtration
Section 2 Centrifugal Separation Equipment
I. Overview of Centrifugal Separation Equipment
Second, centrifugal settling equipment
Three, centrifugal filtration equipment
Four, the amplification of centrifugal separation equipment
Section 3 Ultracentrifugation Technology
First, the principle of ultracentrifugation technology
Second, the classification of ultracentrifugation technology
Third, ultra-high speed centrifugal equipment
Section IV Application of Centrifugal Technology in Biological Separation
I. Matters needing attention in the application of centrifugal technology in biological separation
Second, advantages and disadvantages of centrifugal separation
Third, the choice of centrifuge
Fourth, the application of centrifugation in biological separation
Think about a problem
Chapter 10 concentration, crystallization and drying
The first section evaporation concentration process principle and equipment
I. Evaporation concentration process
Second, evaporation and concentration equipment
Section II Crystallization Process Principle and Equipment
I. Principle of crystallization operation process
Second, the crystallization equipment
Section 3 drying process principle and equipment
First, the principle of drying process
Second, the drying equipment
Think about a problem
Main references