Ozone always inactivates bacteria quickly. Different from other fungicides, ozone can react with the lipid double bond of bacterial cell wall, penetrate into bacteria, act on protein and lipopolysaccharide, change the permeability of cells, and lead to bacterial death. Ozone also acts on nuclear substances in cells, such as purines and pyrimidines in nucleic acids, to destroy DNA.

2 Inactivation mechanism of ozone on virus:

The effect of ozone on virus is firstly that the four polypeptide chains of virus capsid protein are destroyed, especially the protein which forms it. After the bacteriophage was oxidized by ozone, it was observed by electron microscope that its epidermis was broken into many fragments, from which many ribonucleic acids were released, which interfered with its adsorption on sediments. There is no doubt about the thoroughness of ozone sterilization.