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Mathematical analysis Rudin
It's mainly related to your different developer. Different developing agents, due to their different polarities, have a considerable influence on the separation, and also affect the shape of your spot, which has many similarities with liquid chromatography. In fact, normal phase chromatography in liquid chromatography is basically the same as thin layer chromatography. If a general detector is selected for liquid chromatography, it is basically equivalent to thin-layer chromatography, but the separation by liquid chromatography is better than that by thin-layer chromatography, and of course the cost is much higher than it. If the peak shape in liquid chromatography cannot be obtained, the points in thin-layer chromatography will often be out of round, such as trailing or long strips. If the separation is not good, it will often lead to inseparable situations, and of course your results will not be exactly the same. Therefore, choosing a developer depends not only on your Rf value, the shape of the dots, but also on the number of points to watch. Generally speaking, the more points, the better the separation. However, it should be noted that even if some substances are pure, there are many points if the developing agent is not selected well.