1, HIV nucleic acid detection includes HIV- 1 DNA detection and HIV- 1 RNA detection, which directly attacks the RNA or DNA structure of HIV, detects whether there is viral nucleic acid in blood and diagnoses whether there is pathogen infection.

2. Using PCR technology, using amplification reagents commonly used in molecular biology laboratories and self-developed composite primers to cover common HIV strains has high sensitivity.

3. The nested PCR amplification method with secondary amplification is adopted to improve the specificity of detection and reduce the false positive probability.

4. Nucleic acid detection shortens the detection window of HIV to 1 1 day. This nucleic acid detection technology can shorten the detection window of HBV and HCV from 50 days and 72 days to 25 days and 59 days respectively.