1. Toxic particles appear when neutrophils are seriously infected.
2. The pigment part of heme hemoglobin consists of iron atom and protoporphyrin region. 3. The basophils remaining in erythrocytes of spotted erythrocytes are the result of RNA denaturation and precipitation.
4. Neutrophil nuclei move to the left, and neutrophil rod nuclei in peripheral blood increase (granulocytes appear in late, middle and early stages, producing granulocytes). 5. Blood type is the genetic trait of human blood, which refers to the difference of red blood cell antigen.
6.ABO blood group antibodies are generally divided into two categories; Both "natural" antibodies and immune antibodies are produced by immunization. 7. Pathological proteinuria Proteinuria continuously exceeds 0. 15g/d, which is often pathological and is a reliable indicator of renal diseases.
8. Renal tubular proteinuria is caused by normal glomerular filtration membrane, but the normal filtration protein in original urine cannot be completely reabsorbed by renal tubules. 9.bence-jones protein is a light chain monomer or dimer of immunoglobulin, which belongs to incomplete antibody globulin.
It often appears in the urine of patients with dry myeloma and has diagnostic significance. 10. Reticulocytes are immature red blood cells between late immature red blood cells and mature red blood cells.
1 1. Wright dye solution is a composite dye composed of acid dye eosin and basic dye methylene blue, which is dissolved in methanol and dissociated into positively charged methylene blue and negatively charged eosin ions. 12. Atypical lymphocytes are called atypical lymphocytes, which proliferate and undergo some morphological changes under the action of some virus infections or allergens.
13. Degenerated leukocytes show changes such as enlarged cell body, blurred structure, unclear edge, nuclear concentration, swelling or dissolution. 14. More than 3% of the nuclear shift to the right is called nuclear shift to the right.
15. There is LE factor in the blood (body fluid) of patients with s LE, which can depolymerize the DNA of affected leukocytes in vitro to form free isotropic body, which is swallowed by phagocytic leukocytes. 16. Endogenous coagulation system refers to the formation of activated [ⅸ A-PF3-ⅷ-CA] complex of coagulation initiation reaction factor and activation factor ⅹ.
17. Exogenous coagulation system refers to the activation of coagulation factor VII and coagulation factor III, which is manifested by the activation of exogenous coagulation pathway by tissue thromboplastin released after tissue injury. 18. Solid-phase activation of coagulation refers to the process in which a factor contacts a charged surface, that is, it is activated as A (activation process for short).
19. the liquid phase activation of coagulation refers to the rapid activation of a after kallikrein is converted into kallikrein, also known as enzyme activation. 20.Rh blood group red blood cells contain the same antigen as rhesus monkey red blood cells.
2 1. Osmotic pressure difference is the driving force of water reabsorption in vivo, and concentration difference and potential difference are the driving forces of some solutes reabsorption. 22. True diabetes is due to the relative or absolute deficiency of insulin secretion, which reduces the utilization rate of glucose in various tissues in the body.
23. Renal glycosuria refers to the normal blood sugar level, which is caused by the decrease of renal tubule's ability to reabsorb sugar. 24. Hemoglobinuria refers to the phenomenon that hemoglobin produced exceeds the binding capacity of haptoglobin and appears in urine when intravascular hemolysis occurs.
25. Chylouria means that urine contains lymph, and its appearance is milky, which is called chyluria. 26. Protein Error When the sample solution contains protein or polypeptide, serious errors often occur in the colorimetric determination of pH value.
27. Entamoeba histolytica is a recognized pathogenic amoeba. According to the change of its life history, it can be divided into trophoblast stage and cyst stage, which can cause intestinal amoebic dysentery or other amoebic diseases. 28. Exudate is an inflammatory exudate caused by local tissue inflammation. Exudation is a process in which liquid components and cellular components in inflammatory blood vessels enter tissues or body cavities through the blood vessel wall.
29. The water and nutrients in the blood vessels of the filtrate are filtered out through capillaries. This non-inflammatory tissue fluid accumulated in interstitial or body cavity is called filtrate. 30. Hemorheology is a new branch of biomechanics, and it is a science that studies the macroscopic fluidity of blood, the fluidity of blood cells and biochemical components.
The active unit of 4 1. enzyme refers to the consumption of substrate or the output of product per unit time in enzymatic reaction under certain action conditions. 42. The precision of multiple measurements of the same sample, and the closeness of each measurement result to the average value.
43. The level of medical decision-making refers to the concentration of tested components that play a key role in the diagnosis or treatment of diseases. 44. Electrophoresis charged particles move in an electric field.
45. Accuracy refers to the degree to which the measurement result is close to the true value. 46. Electroosmosis is the phenomenon of relative movement of solid support under the action of electric field.
47. Hemolysis When red blood cells are in a hypotonic solution, extracellular fluid enters the cells, resulting in excessive expansion and rupture of red blood cells. This phenomenon is called hemolysis.
2. Basic knowledge of medical examination is urgently needed.
1. Toxic particles appear when neutrophils are seriously infected.
2. The pigment part of heme hemoglobin consists of iron atom and protoporphyrin region. 3. The basophils remaining in erythrocytes of spotted erythrocytes are the result of RNA denaturation and precipitation.
4. Neutrophil nuclei move to the left, and neutrophil rod nuclei in peripheral blood increase (granulocytes appear in late, middle and early stages, producing granulocytes). 5. Blood type is the genetic trait of human blood, which refers to the difference of red blood cell antigen.
6.ABO blood group antibodies are generally divided into two categories; Both "natural" antibodies and immune antibodies are produced by immunization. 7. Pathological proteinuria Proteinuria continuously exceeds 0. 15g/d, which is often pathological and is a reliable indicator of renal diseases.
8. Renal tubular proteinuria is caused by normal glomerular filtration membrane, but the normal filtration protein in original urine cannot be completely reabsorbed by renal tubules. 9.bence-jones protein is a light chain monomer or dimer of immunoglobulin, which belongs to incomplete antibody globulin.
It often appears in the urine of patients with dry myeloma and has diagnostic significance. 10. Reticulocytes are immature red blood cells between late immature red blood cells and mature red blood cells.
1 1. Wright dye solution is a composite dye composed of acid dye eosin and basic dye methylene blue, which is dissolved in methanol and dissociated into positively charged methylene blue and negatively charged eosin ions. 12. Atypical lymphocytes are called atypical lymphocytes, which proliferate and undergo some morphological changes under the action of some virus infections or allergens.
13. Degenerated leukocytes show changes such as enlarged cell body, blurred structure, unclear edge, nuclear concentration, swelling or dissolution. 14. More than 3% of the nuclear shift to the right is called nuclear shift to the right.
15. There is LE factor in the blood (body fluid) of patients with s LE, which can depolymerize the DNA of affected leukocytes in vitro to form free isotropic body, which is swallowed by phagocytic leukocytes. 16. Endogenous coagulation system refers to the formation of activated [ⅸ A-PF3-ⅷ-CA] complex of coagulation initiation reaction factor and activation factor ⅹ.
17. Exogenous coagulation system refers to the activation of coagulation factor VII and coagulation factor III, which is manifested by the activation of exogenous coagulation pathway by tissue thromboplastin released after tissue injury. 18. Solid-phase activation of coagulation refers to the process in which a factor contacts a charged surface, that is, it is activated as A (activation process for short).
19. the liquid phase activation of coagulation refers to the rapid activation of a after kallikrein is converted into kallikrein, also known as enzyme activation. 20.Rh blood group red blood cells contain the same antigen as rhesus monkey red blood cells.
2 1. Osmotic pressure difference is the driving force of water reabsorption in vivo, and concentration difference and potential difference are the driving forces of some solutes reabsorption. 22. True diabetes is due to the relative or absolute deficiency of insulin secretion, which reduces the utilization rate of glucose in various tissues in the body.
23. Renal glycosuria refers to the normal blood sugar level, which is caused by the decrease of renal tubule's ability to reabsorb sugar. 24. Hemoglobinuria refers to the phenomenon that hemoglobin produced exceeds the binding capacity of haptoglobin and appears in urine when intravascular hemolysis occurs.
25. Chylouria means that urine contains lymph, and its appearance is milky, which is called chyluria. 26. Protein Error When the sample solution contains protein or polypeptide, serious errors often occur in the colorimetric determination of pH value.
27. Entamoeba histolytica is a recognized pathogenic amoeba. According to the change of its life history, it can be divided into trophoblast stage and cyst stage, which can cause intestinal amoebic dysentery or other amoebic diseases. 28. Exudate is an inflammatory exudate caused by local tissue inflammation. Exudation is a process in which liquid components and cellular components in inflammatory blood vessels enter tissues or body cavities through the blood vessel wall.
29. The water and nutrients in the blood vessels of the filtrate are filtered out through capillaries. This non-inflammatory tissue fluid accumulated in interstitial or body cavity is called filtrate. 30. Hemorheology is a new branch of biomechanics, and it is a science that studies the macroscopic fluidity of blood, the fluidity of blood cells and biochemical components.
The active unit of 4 1. enzyme refers to the consumption of substrate or the output of product per unit time in enzymatic reaction under certain action conditions. 42. The precision of multiple measurements of the same sample, and the closeness of each measurement result to the average value.
43. The level of medical decision-making refers to the concentration of tested components that play a key role in the diagnosis or treatment of diseases. 44. Electrophoresis charged particles move in an electric field.
45. Accuracy refers to the degree to which the measurement result is close to the true value. 46. Electroosmosis is the phenomenon of relative movement of solid support under the action of electric field.
47. Hemolysis When red blood cells are in a hypotonic solution, extracellular fluid enters the cells, resulting in excessive expansion and rupture of red blood cells. This phenomenon is called hemolysis.
3.
Hello, interviewers!
Medical examination is to provide reliable diagnostic basis for clinical medical treatment, directly serve patients and serve clinic. Therefore, I take the professional ethics service of medical laboratory personnel as the content of today's speech.
First of all, we should correctly understand the position and function of medical laboratory. With the development of modern medical technology, the medical laboratory is constantly updated, and its position and function are paid more and more attention by clinic. Thereby providing reliable basis for disease diagnosis, curative effect monitoring and prognosis judgment, and playing an irreplaceable role in clinical medicine.
Therefore, we should constantly absorb and apply new knowledge and constantly update the methods and items of clinical examination, which makes the role of clinical examination more and more important in medicine. At the same time, we should not only ensure the quality, but also require more and deeper testing items. As inspectors, we should grasp the new theories, knowledge and technology of medical inspection in time, pay attention to the evaluation, screening and acceptance of related inspection items and methods, and strive to improve the accuracy of inspection items, so as to better serve the clinic and improve our status. To give full play to the subjective initiative of inspectors, we should obey the needs of clinicians and conduct careful inspection according to the inspection application form. If the samples submitted for inspection do not meet the requirements, they should be collected again in time and operated according to the operating procedures, and the report should not be issued blindly, otherwise it will affect the diagnosis and treatment of diseases and bring pain to patients. Therefore, in the spirit of serving patients wholeheartedly and under the premise of obeying the clinic, the inspectors should find the problems existing in the inspection application form or clinical samples as soon as possible, modify them in time and reduce errors, which is the embodiment of the noble medical ethics of the inspectors.
Second, we must abide by professional ethics. Clinical inspectors should restrain their work according to medical theory and moral norms. Give full play to the advantages and characteristics of this major, actively provide accurate diagnosis basis for clinical departments, and serve the people wholeheartedly. It is necessary to strictly operate the procedures to ensure the inspection quality. The operating rules of clinical laboratory technology are the requirements to standardize the operating methods, steps and procedures of various laboratory technologies. It is also an important measure to ensure the quality of inspection. However, in daily work, there are irregular operations, such as the classification of white blood cells without oil mirror, bilirubin and other biochemical items to determine whether it is normal by color, and so on, relying too much on instruments. If there is a urine analyzer, urine microscopy will not be done, and if there is a blood cell analyzer with classification, microscopic classification will not be done. This irregular practice will affect the quality of inspection. Clinical laboratory personnel should pay attention to their professional ethics and must use clinical laboratory theory and ethics to regulate their behavior. Only in this way can we improve the overall quality of clinical laboratory team, further improve the status and role of clinical laboratory, develop new technologies, methods and projects of clinical laboratory, and continuously benefit patients and serve mankind.
In a word, medical laboratory personnel, while possessing noble professional ethics and good professional quality, firmly establish the idea of taking patient service as the center, keep the spirit of continuous progress, study professional knowledge hard, improve the standardization of work, better serve the clinic, and make medical laboratory work to a new level.
4. Summary of clinical practice in clinical laboratory.
Original publisher: spfgg5930
Laboratory 1 biochemical laboratory internship summary, the summary time of laboratory biochemical laboratory internship is like a blink of an eye. In a blink of an eye, we have moved from school to society. The scene when we were at school yesterday was vivid, but today we have left school and come to another strange place to study and work. It has been more than half a month since I came to the First People's Hospital of xx. During the period, I had a lot of insights and feelings, and learned a lot of knowledge and the truth of being a man. On June 28th, we went to work. We are wearing white coats. Under the leadership of Director Li Qixin, we probably know the positions of various departments in the clinical laboratory. Shuttling between different departments, I can see the expectations of patients in white coats. After a brief introduction, we started to work, and the work came faster than I expected. We went to work directly without any training, which made me a little worried. My first department is in the biochemical room. After a brief introduction, I started my normal work. At first, I signed and numbered the specimens. It seems that this work is very simple, but inadvertently, I asked the teacher many times whether this specimen should be input into that instrument, whether it is bidirectional or unidirectional. The teacher took pains to answer me, which made me feel extremely warm. I can imagine that in such an environment, I can learn the knowledge and technology I want. This fully shows that the inspection work is not simple. I will study harder, seriously and modestly. Working in the department, patients will come up from time to time and ask: doctor, is this project tested here? I am proud and happy to hear the patient's voice, doctor. However, at the beginning, the division of labor among various departments in the laboratory was not very clear, and I couldn't answer the patient's questions. At this time, I was very unhappy.
5.
Both serum iron and serum ferritin are used to detect iron deficiency anemia, but they are different: iron exists in the body in the form of protein binding, and the distribution of iron mainly has two parts: one is running serum iron, which performs physiological functions; The second is to store iron that does not perform physiological functions, mainly ferritin and hemosiderin.
1, serum iron, also known as transferrin, is a complex of β 1 globulin in plasma. The role of serum iron is to transport iron to red blood cells to produce hemoglobin, so it mainly exists in the form of heme. Therefore, iron deficiency will cause the decrease of serum iron, that is, the synthesis of transferrin and hemoglobin and anemia, so the determination of serum iron can diagnose iron deficiency anemia.
2. Serum ferritin is the storage of iron. It is an iron-containing protein complex, which consists of ferritin and iron core Fe3, and has great iron storage capacity. Serum ferritin can reflect the total amount of iron stored in the body and the nutritional status of iron, which is the most sensitive index to check iron deficiency in the body and is used for the diagnosis of iron deficiency anemia in clinic.
The above two items, serum iron reflects transported iron and serum ferritin reflects stored iron, can be combined to better diagnose iron deficiency anemia.
6. What should medical laboratory technology learn?
Specialized content of medical laboratory technology: This major trains senior medical professionals with basic theoretical knowledge and basic ability of basic medicine, clinical medicine and medical laboratory, who can engage in medical laboratory and medical laboratory work in hospitals, blood stations and epidemic prevention departments at all levels.
Students should master the basic theoretical knowledge and experimental operation ability in basic medicine, clinical medicine, medical examination and experimental diagnosis, and at the same time fully grasp the knowledge of natural science and humanities and social sciences. After graduation, I can engage in clinical laboratory, medical laboratory teaching and scientific research.
Curriculum of laboratory technology specialty in medical college: biochemistry, medical statistics, analytical chemistry, laboratory instruments, physiology, pathology, parasitology and testing, microbiology and testing, immunology and testing, hematology testing, clinical biochemistry and testing, etc.
Extended data:
Requirements of laboratory technology specialty in specialized medicine;
1, master the basic theoretical knowledge and experimental skills of medical chemistry, medical physics and basic medicine;
2, master the basic knowledge of clinical disease diagnosis;
3, master the basic theoretical knowledge and experimental operation skills of clinical medical examination;
4, master the basic knowledge and skills of mathematical statistics and computer application;
5, master the structure and operation skills of various commonly used medical inspection and analysis instruments;
6, master the basic knowledge and skills related to health inspection;
7, master the preliminary ability of clinical medical inspection and scientific research work.