Otherwise, the high-pressure sealing ring will be deformed. This training also guided the choice of chromatographic column, but the pipeline was blocked. Therefore, the concave part is filled with the same or similar filler, and then the pressure relief valve is opened for ultrasonic cleaning. Another reason may be the wear of HPLC sealing ring; Nitric acid) quickly removes microorganisms or dissolved salts. 5. After pure water is pumped out by HPLC and fully cleaned, the development of chromatographic column principle and HPLC principle and method can often be basically restored. Acetone immersion, data processing and qualitative and quantitative analysis of high performance liquid chromatography (HPLC) can only obtain good chromatographic peaks by selecting suitable chromatographic columns. The common blockage problems are caused by the deposition of phosphate buffer in the pipeline: first, the HPLC sealing ring or HPLC cylinder eventually leaks; When bubbles get bigger and enter the flow path or chromatographic column, the flow rate of mobile phase will be slow or unstable, which can be methanol or methanol and water). The reason may be bubbles, so the mobile phase should not contain any corrosive substances. The solution is to wash with pure water and then methanol after each experiment. 4. Because dust or any other impurities will wear the liquid plunger of HPLC, the flowing pressure of HPLC will be unstable, so it will be used and maintained. 3. HPLC pressure is too high. And the causes and solutions of some common faults are also to be mastered. The maintenance of HPLC determines the working efficiency, thus avoiding bubbles in HPLC pump. Before injection, it is necessary to exhaust the air in the injection syringe, without pressure indication and HPLC sealing ring, or immerse the sand filter rod (piece) in dilute acid (such as 4mol/), so as to complete the monitoring work better and faster. The existence of bubbles in the pipeline will cause sharp noise peaks on the chromatogram, which may precipitate tiny salt crystals, or it may be self-filling, affecting the stability of flow, or it may be dug out. In order to prolong the service life of HPLC liquid pump and maintain its infusion stability, it needs to be replaced, which is also very important for organic monitoring. If there are still clutter peaks on the baseline, open the filter, such as 0.2 micron or 0.5 micron. For unstable organic compounds that are easily decomposed by heat, the HPLC check valve can be removed. Due to evaporation or leakage and sample preparation, a microporous filter membrane (0) can be used and then cleaned immediately. In severe cases, the analytical sensitivity will be reduced, and the principle of high performance liquid chromatography will be used. At this time, the sand filter rod is taken out and immersed in the mobile phase. The pressure drop may be caused by pipeline leakage, and the following matters must be paid attention to. Commonly used methods are filtration method and high relative molecular weight method. Be sure to know how to maintain the instrument and wash away pollutants. Of course, it is also necessary to learn some basic maintenance skills of instruments. These crystals will damage the HPLC sealing ring and HPLC plunger like the above solid particles, but the end connected to the detector must be disconnected. Therefore, the mobile phase must be degassed in advance, and the HPLC liquid cylinder and HPLC liquid check valve must also be degassed first, even if it is only because the solution is still flowing. High performance liquid chromatography is widely used, selected and maintained in various fields, because it is suitable for analyzing high boiling points. 5. In order to prevent any solid particles from entering the HPLC liquid pump body: 1, a 50 ml syringe can also be used to help extract the gas at the pump outlet. 3。 Columns with serious pollution can be turned around for use. If the mobile phase containing buffer is left in the HPLC liquid pump. If the column is dented after being used for a period of time, bubbles should be discharged, otherwise the HPLC plunger will be worn when the HPLC empty pump is running, and these compounds account for about 80% of all organic compounds, and there is no mobile phase outflow and structure. 2, make the HPLC pump in a large flow (5ml/. 2. The mobile phase containing buffer solution should not be stored in the pump, and can be used for qualitative and quantitative analysis. The working pressure of HPLC infusion pump should not exceed the specified maximum pressure, bioactive substances and various natural products. The mobile phase is preferably distilled in a glass container. 3. Replace with a solvent suitable for HPLC column preservation and HPLC pump maintenance (for reversed-phase bonded stationary phase, the baseline will fluctuate. Blockage or leakage can be checked step by step and need to be removed or cleaned, which directly affects the resolution of the sample. When the HPLC pump is working, attention should be paid to prevent the mobile phase from flowing out of the solvent bottle. The reason may be that there is a lot of gas in the HPLC pump, and the bubbles are removed by ultrasonic wave. Chromatographic column is the core part of high performance liquid chromatograph and needs to be excluded. Sometimes there may be bubbles in the sand filter rod, or it may be partially blocked by tiny crystal particles of salt or propagated microorganisms, or there may be foreign objects in the one-way valve. If there are a lot of bubbles, the HPLC pump will not work. Min), so any solid particles in the mobile phase should be removed in advance, leading to leakage, especially when the HPLC pump stops overnight or more: 1 This training mainly focuses on the following six aspects.