For nucleic acid detection in novel coronavirus, samples were collected according to the "Sample Requirements" section of the kit instructions. Routine sample types include throat swab, nasal swab, sputum, bronchial lavage fluid, alveolar lavage fluid and so on.

After obtaining patient samples, it is necessary to test them as soon as possible. If the samples that need to be transported cannot be tested immediately, they shall be packaged at low temperature according to the requirements of the instructions and sent to a special testing institution for testing. After receiving the sample, the testing institution shall extract nucleic acid from the sample, and the nucleic acid extraction reagent shall use the nucleic acid extraction kit specified in the approved product specification.

Viral RNA needs to be reverse transcribed into cDNA before amplification and detection. PCR amplification and detection should use the fluorescent quantitative PCR instrument specified in the approved product manual. The Ct value of the sample obtained by fluorescence quantitative PCR can determine whether the patient sample contains novel coronavirus.

Extended data

Principle of nucleic acid detection

All living things except prions contain nucleic acids, including deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). Novel coronavirus is a virus containing only RNA, and the specific RNA sequence in the virus is a sign to distinguish the virus from other pathogens. After novel coronavirus appeared, scientists in China completed the analysis of the whole genome sequence of novel coronavirus in a short time.

Compared with other species' genome sequences, novel coronavirus's specific nucleic acid sequence was found. If novel coronavirus's specific nucleic acid sequence can be detected in the patient's sample during the clinical experiment, it should be suggested that the patient may be infected by novel coronavirus.

Baidu Encyclopedia-Accounting Detection Method