Aseptic technology mainly refers to a series of operation methods and related measures to control or prevent the pollution and interference of various miscellaneous bacteria in production, including aseptic environment facilities, aseptic operation equipment and aseptic operation. , collectively referred to as aseptic technology. Aseptic technology is a complete operating system. If any part of it belongs to non-aseptic technical operation, then the other parts, even aseptic technical operation, are completely meaningless, which will lead to the failure of the whole production process. Therefore, every link in the production should be carefully operated according to the requirements and cannot be taken lightly.

(1) mixing and bagging

It is required that all components of the culture medium have no mildew, good dispersibility, cleanliness and hygiene. After mixing with water, water is required to fully penetrate into the material without caking. Avoid the damage and micropores caused by various factors in bagging.

(2) sterilization process

The sterilization operation procedures should be strictly followed to ensure the sterilization time and temperature, eliminate the sterilization dead angle and ensure thorough sterilization.

(3) Sterilization in aseptic environment

Aseptic environment refers to a space in which the number of microorganisms is reduced to a minimum and is close to aseptic by physical or chemical methods in a controllable space. Strict sterility is difficult to achieve. Inoculation room, inoculation box, clean workbench, etc. Can be made into such a space. Inoculation room, inoculation box and other inoculation places require sterility, and the simple and effective method is drug fumigation. The degree of sterility after disinfection in the inoculation environment should meet the prescribed standards.

(4) Inoculation

Aseptic operation generally refers to the routine operation method that can prevent contamination and interference of miscellaneous bacteria in the process of inoculation in an aseptic environment. With good and qualified aseptic conditions, if the operation is not carefully standardized, it is easy to cause bacterial infection. Therefore, we should do well: ① personnel training. All operators must undergo strict technical training in advance, firmly establish the concept of sterility, and master the aseptic operation procedures before taking up their posts. (2) Strict aseptic operation is carried out during inoculation, which meets the requirements of aseptic operation procedures.

(5) Transport links

In the process of transporting sterilized bottles and bags to the culture site, they should be handled with care to avoid loose seals and damage to plastic bags. Many producers often ignore this link.

(6) cultivation link

The fungus bag culture site should be disinfected according to the requirements, and the contaminated fungus bottles and fungus bags should be inspected frequently during the culture process and treated in time to prevent the spread of miscellaneous bacteria.