7. The laboratory operating rules on how to deal with leakage should be posted in the leakage laboratory, and everyone who uses the laboratory should read and understand these rules. Once the bio-hazardous substances in the bio-safety cabinet overflow, they should be cleaned immediately under working conditions. Effective disinfectants should be used, and the generation of aerosol should be reduced as much as possible during the treatment. All materials that come into contact with spilled substances should be disinfected and/or autoclaved.
8. Certification: After being installed at regular intervals in a season, the operational performance and integrity of each biosafety cabinet should be certified by qualified professionals according to the manufacturer's instructions to check whether it meets the national and international performance standards. The evaluation of the protective effect of the safety cabinet should include testing the integrity of the safety cabinet, the leakage of HEPA filter, the downward airflow speed, the frontal airflow speed, the negative pressure/air exchange times, the smoke type of the airflow and the alarm and interlock system.
You can also choose to test leakage, illumination, ultraviolet intensity, noise level and vibration. When conducting these tests, inspectors should be specially trained and use special technology and equipment. Testing by qualified professionals is strongly recommended.
9. Cleaning and disinfection Because the residual culture medium may make microorganisms grow and multiply, at the end of the experiment, all items in the biosafety cabinet, including equipment, should be clearly contaminated on the surface and taken out of the biosafety cabinet.
Before and after each use, the pollution on the inner surface of the biosafety cabinet should be removed. The countertop and inner wall should be wiped with disinfectant, and the disinfectant used should be able to kill any microorganisms that may be found in the biosafety cabinet. At the end of the daily test, the table, periphery, inside and outside of the glass should be wiped to remove surface contamination. When it is effective for the target organism, it can be disinfected with bleach or 70% alcohol. After using corrosive disinfectants such as bleach, they must also be used.
It is recommended to keep the safety cabinet running at all times. If it is to be shut down, it should be shut down after running for 5 minutes to purify the gas inside.
10. Cleaning up pollution Before moving or replacing the filter, the biosafety cabinet must be cleaned. The most common method is fumigation with formaldehyde steam. The pollution of biosafety cabinets should be cleaned by qualified professionals.
1 1. Personal protective equipment Personal protective clothing should be worn when using biosafety cabinets. Ordinary test clothes can be worn when operating at the first and second biosafety levels. Isolation gown, the reverse test reinforced in front, has better protection effect, and should be used in the operation of biosafety level III and IV (except the protective clothing laboratory). Gloves should be put on the outside of isolation gown and can be tied with elastic bands.
12. The alarm can be equipped with a biosafety cabinet by selecting one of two kinds of alarms. Window alarms can only be installed on safety cabinets with sliding windows. When an alarm is given, it means that the operator has moved the sliding window to an inappropriate position. When handling this alarm, just move the sliding window to the appropriate position. When the air flow alarm gives an alarm, it means that the normal air flow pattern of the safety cabinet is disturbed. Operators or articles are immediately in danger. When the airflow alarm sounds, they should stop working immediately and notify the laboratory director. The manufacturer's instruction manual will provide more detailed information, which should also be included in the use training of biosafety cabinets.