Virus name: frangipani mosaic virus (FrMV). Synonyms of virus: Temple.tree.mosaic.virus, Champa.mosaic.virus purification: (1) report method of francki et al. (197 1). The diseased leaves were homogenized with 1.5 (v/w) .0.2 mol/L Na2HPO4, clarified with activated carbon and DEAE fiber, filtered with diacetyl plastic, centrifuged at 44000r/min for 90min, and the virus was precipitated. Suspend the precipitate in distilled water, add equal amount of chloroform for emulsification, centrifuge at 65438±02000 r/min, take water phase, centrifuge for 0.30 min, and then repeat chloroform extraction for precipitation. ② Valma, Gibbs. & amp。 Woods. (1970). Add 2ml/g neutral phosphate-ascorbic acid buffer (0. 1mol/L.Na2PO4 and 0.05 mol/L.ascorbic acid, mix well), grind, add 1/4 chloroform to the extract, emulsify, centrifuge at 8000r/min. The granules are rod-shaped, 297 ~ 300 nm× 17 nm, and there are also short granules in the preparation (Francki.et.al, 197 1). The virus contains precipitated components. The sedimentation coefficient is 188S. A260/A280 is 1.2 1. Help the virus. ② nucleic acid. Single stranded RNA, accounting for about 5%. 3 protein. It contains 95% capsid protein of Adel strain, and each subunit contains about 158. Amino acid residue: Ala,14; Arg， 1 1； Asx， 17； Cys， 1； Glx， 16； Gly，9； His， 1； Ile， 1 1； Leu， 13； Lys，4； Encountered, 0; Phe，7； Pro，4； Ser， 14； Thr， 13； Trp，5； Tyr，5； Val， 13。 (Frankie et al., 197 1). The capsid protein composition of Salmonella cactus virus is very similar to FrMV. Strain: There are three strains: Adelaide.strain.(Adel) and Allahabad. And Delhi. (del). Strains are differentiated according to symptoms. At about 22℃, Datura stramonium was inoculated with Adel, and the inoculated leaves produced fuzzy chlorosis spots. Local necrosis spots appeared after Ald; Followed by del. First, the spots faded locally, and then turned black. At 35℃, the symptoms developed rapidly and greatly: Adel showed local necrotic spots; Ald local chlorosis halo spots or ring spots; Del locally dilated black circle spots, systemic vein necrosis and leaf margin necrosis. When inoculated with the tobacco variety Virginia. Only gold. The Delaware River shows symptoms, showing local chlorosis and necrotic spots, while at 35℃, all symptoms appear. Adel showed fuzzy necrotic ring spots on inoculated leaves, Ald showed bright necrotic ring spots, and del showed large local ring spots and large ring spots in parietal lobe system.