Opened up a new scientific field of serology, 1892 returned to Japan. With the help of Fukuzawa Yukichi and others, he set up a private infectious disease research institute under the Great Japan Health Association, and served as its director, taking the lead in carrying out the research on infectious diseases and bacteriology in Japan. 1893, in order to accommodate tuberculosis patients for treatment, a health park was established and served as the director. During the plague epidemic in Hong Kong from 65438 to 0894, he was ordered to go to the epidemic area in May, and conducted a field investigation with Yin Tong and others in Qingshan. In June, he and the Swiss bacteriologist Alexander Enmir-John Yersin isolated the pathogenic factor of the disease, Yersinia pestis. From 65438 to 0897, Beijing Institute of Bacteriology was established and began to engage in the research of dysentery bacteriology. 1898, Japanese bacteriologist Shigejie isolated Shigella dysenteriae from the feces of dysentery patients according to his suggestion, which is now (Shigella). 19 14, his research institute was changed to a national research institute, and when it was handed over to Imperial University of Tokyo for management, he resigned as the director. 19 17 set up a medical department in Keio University as a minister. From 1923, he became the president of the Japanese Medical Association, and 1924 was made a baron. He has become an academician of the Bachelor's College, a member of the Royal Society and an honorary member of some American and German societies. He is the editor-in-chief of the Journal of Experimental Medicine of Beijing University of Science and Technology. He has published many papers on bacteriology. 1931June 13 died in the middle city of gunma county. Keio University established the Doctor Medical Library of Beijing University in 1937 to commemorate him.