First, the main tasks of clinical biochemical tests

Second, a brief history of the development of clinical biochemical tests

Thirdly, the modernization of clinical biochemical tests.

Four. The main content and usage of this book

Chapter II Basic Knowledge of Clinical Biochemical Tests

Section 1 Application Methods and Reports of Clinical Biochemical Tests

I. Individual inspection and joint inspection

Second, the report form

Section 2 Quality Management Elements of Clinical Biochemical Tests

A, clinical biochemical test process

Second, the elements of quality management before inspection

Three, the quality management elements in the inspection

Four, quality management elements after inspection

Section 3 Laboratory Information Management System

I. Overview of Laboratory Information Management System

Second, the improvement and maintenance of laboratory information management system

Chapter III Common Techniques of Clinical Biochemical Tests

The first section spectral analysis technology

First, the basic principle of spectral analysis technology

Second, the application of spectral analysis technology in clinical biochemical tests

Third, the influencing factors of spectral analysis technology

Section 2 Electrochemical Analysis Technology

First, the basic principle of electrochemical analysis technology

Second, the application of electrode analysis method in clinical examination

Third, the influencing factors of electrode analysis method

Section III Dry Chemical Analysis Technology

First, the basic principle of dry chemical analysis technology

Second, the application of dry chemical analysis technology in clinical examination

Third, the influencing factors of dry chemical analysis technology

Section 4 Electrophoretic Analysis Technology

First, the basic principle of electrophoretic analysis

Second, the commonly used electrophoresis analysis technology

Three, automatic electrophoresis analysis technology

Four. Clinical application of electrophoretic analysis technology

Section 5 Gene amplification technology

First, the basic principle of PCR reaction

Second, the basic principle of fluorescence quantitative PCR

Third, the clinical application of PCR reaction technology

Chapter IV Automatic Biochemical Analysis Technology

Section 1 Overview

A, semi-automatic biochemical analyzer

Second, the automatic biochemical analyzer

Section 2 Parameter Setting and Calibration of Biochemical Analyzer

I. Explanation of Nouns

Second, the parameter setting of biochemical analyzer

Third, the detection system and quantity traceability

Fourth, the calibration of biochemical analyzer

Section III Verification, Confirmation and Evaluation of Test System Performance

The fourth section of the continuous effectiveness of the testing system

First of all, the operating environment of the detection system should meet the requirements continuously.

Second, the indicators of the detection system should continue to meet the requirements.

Three. Verification and verification of biochemical analyzer

Section 5 Selection and Evaluation of Biochemical Analyzer Kit

First of all, the type of toolkit

Second, buy biochemical kits.

Thirdly, the use and performance evaluation of the kit.

Chapter V Evaluation and Verification of Detection System

Section 1 Scope of Application of Clinical Laboratory Performance Evaluation

First, the classification of experimental methods

Second, the scope of application of clinical laboratory performance evaluation

Section 2 Basic Methods of Performance Evaluation and Verification

I. Accuracy evaluation

Second, the accuracy evaluation

Third, accuracy verification.

Fourthly, linear range evaluation.

Evaluation of verb (abbreviation of verb) carrying pollution rate

Verification of biological reference interval of intransitive verbs

Section III Indoor and Inter-room Comparative Experiments

Chapter VI Enzymology Analysis Technology

Section 1 Basic knowledge of enzyme analysis

I. Overview

Second, the enzymatic reaction process

Three. kinetics of enzyme-catalyzed reactions

Section 2 Determination Method of Enzyme Activity

First, the timing method

Second, the continuous monitoring method

Section III Enzymatic Analysis Techniques of Metabolites

I. Tool enzyme

Second, single enzyme reaction and enzyme coupling reaction technology

Section 4 Isozyme Analysis

First, the mechanism of isozyme production

Second, isozyme analysis.

Section 5 Clinical application of diagnostic enzymology

First, the source and change mechanism of enzymes in blood

Second, the clinical commonly used serum enzymology analysis

Chapter VII protein Inspection

Section 1 Overview

I. Classification and function of plasma proteins.

Second, several major protein in plasma.

Third, the changes of plasma protein during the disease.

Protein inspection in the second quarter

First, the determination of serum total protein

Second, the determination of serum albumin

Third, the determination of serum globulin

Four, cerebrospinal fluid protein determination.

Five, serum protein electrophoresis.

Six, urine protein electrophoresis analysis.

Chapter VIII Examination of Abnormal Glucose Metabolism

Section 1 Overview

I. Regulation of blood sugar and blood sugar concentration

Second, hyperglycemia and diabetes.

Third, other sugar metabolism disorders.

Section 2 Tests Related to Glucose Metabolism

A, serum (plasma) glucose determination

Second, oral glucose tolerance test

Third, the determination of glycosylated hemoglobin

Fourth, the determination of glycosylated serum protein

Five, insulin and insulin release test

Six, C peptide and C peptide release test

Seven, urine sugar determination

Eight, cerebrospinal fluid glucose determination

Chapter IX Inspection of Lipid Metabolism and Lipid Metabolism Disorder

Section 1 Overview

I. Blood lipids and plasma lipoproteins

Second, lipoprotein metabolism disorder and its relationship with atherosclerosis

Section 2 Determination of Blood Lipids, Lipoprotein and Apolipoprotein

A, serum (plasma) static test

Second, the determination of serum (plasma) total cholesterol

Determination of serum (plasma) triacylglycerol

Four, serum (plasma) lipoprotein determination

Determination of serum apolipoprotein

Chapter 10 Inspection of Sodium, Potassium, Chlorine and Acid-base Balance

Section 1 Metabolism and Detection of Sodium, Potassium and Chlorine

Distribution and Function of Water and Electrolyte in Body Fluid

Two, sodium, chlorine metabolism and its balance disorder

Third, potassium metabolism and its balance disorder

Four. Determination of sodium, potassium and chlorine

The second quarter acid-base balance and blood gas analysis

First, the transport of gas in the blood.

Second, the method of blood gas analysis

Three, commonly used indicators and parameters of blood gas analysis

Four, the classification and judgment of acid-base balance disorder

Chapter II XI Inspection of Calcium, Phosphorus, Magnesium and Trace Elements

Section 1 Overview

I. metabolism and regulation of calcium, phosphorus and magnesium

Second, the physiological functions of calcium, phosphorus and magnesium

Three. Trace elements and diseases

……

Chapter XII Examination of Hepatobiliary Diseases

Chapter XIII Renal Function Examination and Early Renal Injury

Chapter 14 Detection of Myocardial Damage Markers

Chapter 15 Examination of Pancreatic Diseases

Chapter XVI Examination of Endocrine Diseases

Chapter XVII Biochemical Examination of Pregnancy and Newborns

Chapter 18 Detection of Therapeutic Drug Concentration

Chapter 19 Indoor quality control and inter-room quality evaluation

Appendix Common Reference Interval of Biochemical Test

Chinese-English index

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