The first chapter is the basic knowledge and technology of pharmacological experiments.
First, the purpose and requirements of pharmacological experiment course
Second, the mastery and management methods of experimental animals
Experiment 1. 1 capture and administration methods of mice.
Experiment 1.2 methods of preservation and administration in rats
Experiment 1.3 methods of preservation and administration in rabbits
Methods of catching and managing guinea pigs
Experiment 1.4 methods of dog traction and administration
Third, anesthesia of commonly used experimental animals
Four, blood collection methods of experimental animals
The second chapter is the general pharmacological experiment.
First, the role of drugs on the body (pathogen)
Experiment 2. 1 local and systemic effects of drugs
Experiment 2.2 Indirect Effects of Drugs
Second, the role of the body in drugs
Experiment 2.3 Estimation of Pharmacokinetic Parameters of Sodium Ausulfonate
Experiment 2.4 Determination of Drug Plasma Protein Binding Rate
Third, the factors that affect the action of drugs.
Experiment 2.5 Factors Affecting the Action of Drugs
First, the influence of dosage form on drug action
Second, the influence of the route of administration on the drug action.
Third, the influence of liver function on drug action.
Chapter III Drug Experiment of Central Nervous System
First, general anesthesia experiment
Experiment 3. 1 Activity Determination of Volatile Liquid Anesthetics
Second, sedation and hypnosis experiment
Experiment 3.2 Comparison of the effects of barbiturates
Experiment 3.3 Synergistic effect of sedatives and hypnotics and anti-central stimulants
Experiment 3.4 Effects of Drugs on Spontaneous Activity of Animals
Third, the experiment of antiepileptic drugs and anticonvulsants.
Experiment 3.5 Anticonvulsive Effects of Drugs
Experiment 3.6 Effects of Drugs on Convulsions Caused by Central Excitatory Drugs
Fourth, the experiment of antipsychotic drugs
Experiment 3.7 Stability of Chlorpromazine
Experiment 3.8 Effect of chlorpromazine on basal metabolism in mice
Experiment 3.9 Analgesic Effect of Drugs (Hot Plate Method)
Experiment 3.9 B Analgesic Effect of Drugs (Chemical Stimulation Method)
Five, the central doping experiment
Experiment 3. 10 Comparison of convulsion types and action sites between strychnine and tetrandrine.
Experiment 3. 1l nikethamide antagonizes the respiratory inhibition of morphine.
Experiment 3. Effect of12 nimodipine on memory acquisition in mice
Chapter IV Efferent Nervous System Drug Experiment
Experiment 4. 1 Effects of efferent neurodrugs on blood pressure, intestinal peristalsis and gland secretion in anesthetized dogs.
Experiment 4.2 Effects of drugs on isolated rabbit aortic strips
Experiment 4.3 Effects of Drugs on Isolated Intestines
Experiment 4.4 Effects of drugs on vas deferens in guinea pigs in vivo.
4.5 organophosphorus drug poisoning and rescue experiment
Experiment 4.6 Comparison of surface anesthesia effects between procaine and tetracaine
Chapter V Drug Experiment of Visceral System
First, the drug experiment of treating cardiac insufficiency
Experiment 5. Effect of1cardiac glycoside on isolated heart (Rana chensinensis heart perfusion method)
Experiment 5.2 Effects of drugs on contraction and relaxation characteristics of isolated papillary muscles
Experiment 5.3 Effects of Drugs on Left Ventricular Function and Hemodynamics in Rats
Experiment 5.4 The effect of drugs on cardiac pumping function was measured by impedance cardiogram.
Second, antiarrhythmic drug experiment
Experiment 5.5 Quinidine antagonizes aconitine-induced arrhythmia in rats.
Experiment 5.6 Effects of Drugs on the Threshold of Electrically Induced Ventricular Fibrillation in Rabbits
Experiment 5.7 Therapeutic effect of lidocaine on arrhythmia induced by barium chloride
Experiment 5.8 Antagonistic effect of lidocaine on ouabain-induced arrhythmia
Experiment 5.9 Effects of Drugs on Arrhythmia after Ischemia-reperfusion in Rats
Experiment 5. Effects of10 drugs on effective refractory period and contractility of isolated atrial muscle.
Third, the experiment of anti-myocardial ischemia drugs
Experiment 5. 1 1 Myocardial infarction caused by ligating rabbit coronary artery
Experiment 5. Effects of12 drugs on myocardial contractility and coronary flow in isolated guinea pig hearts.
Experiment 5. 13 nitrotetrazolium blue staining method was used to determine the range of myocardial infarction.
Experiment 5. 14 Effect of Danshen Injection on Pituitrin-induced Myocardial Ischemia in Rabbits
Experiment 5. 15 determination of blood lipid
Fourthly, the experiment of antihypertensive drugs.
Experiment 5. Analysis of hypotensive mechanism of16 hexane quaternary ammonium salt
Experiment 5. 17 vascular perfusion in isolated rabbit ears
Experiment 5. 18 Preparation of Rat Renal Artery Stenotic Hypertension Model
Five, diuretic and dehydration drug experiment
Experiment 5. 19 diuretic effects of furosemide and hypertonic glucose on rabbits
Experiment 5.20 Diuretic Effect of Hydrochlorothiazide on Rats
Experiment 5.2 1 Determination of Sodium, Potassium and Chlorine in Urine
Six, antitussive drugs, expectorant and antiasthmatic drugs experiment
Experiment 5.22 The antitussive effect of codeine on ammonia-induced cough in mice.
Experiment 5.23 The antitussive effect of codeine on cough caused by electrical stimulation of superior laryngeal nerve in cats.
Experiment 5.24 Effect of Radix Polygalae Decoction on Phenol Red Secretion in Trachea of Mice
Experiment 5.25 Antiasthmatic effect of aminophylline on histamine-acetylcholine-induced asthma in guinea pigs.
Experiment 5.26 Effects of Drugs on Isolated Guinea Pig Trachea
Seven, digestive system drug experiment
Experiment 5.27 Effects of drugs on gastrointestinal peristalsis
Experiment 5.28 Preventive and Therapeutic Effects of Drugs on Experimental Gastric Ulcer
Experiment 5.29 cholagogic effect of dehydrocholic acid on rats
Eight, procoagulant and anticoagulant experiments
Experiment 5.30 Anticoagulant Effect of Drugs in Vitro
Experiment 5.3l The procoagulant effect of drugs
Nine, antiplatelet drugs
Experiment 5.32 Determination of Platelet Adhesion
Experiment 5.33 platelet aggregation test,
Experiment 5.34 "Arteriovenous Bypass Thrombosis Experiment"
Experiment 5.35 Bioassay of Thromboxane TxA2 Produced by Platelets
Experiment 5.36 Competitive antagonism of diphenhydramine against histamine and pA: value
Chapter VI Anti-inflammatory Drug Experiment
Experiment 6. Effect of1hydrocortisone on peritoneal capillary permeability in mice
Experiment 6.2 Effect of indomethacin on ear swelling of croton oil in mice
Experiment 6.3 Effect of indomethacin on carrageenan-induced paw swelling in rats
Experiment 6.4 Effect of Dexamethasone on Granuloma in Rats
Experiment 6.5 Effects of Drugs on Experimental Pleurisy
Experiment 6.6 Effects of Drugs on Immune Inflammation
Experiment 6.7 Anti-inflammatory Experiment of Bilateral Adrenalectomy in Rats
Experiment 6.8 Determination of lysosomal enzyme activity
Radioimmunoassay in Experiment 6.9,
Experiment 6. Determination of glucocorticoid receptor in10 leukocytes
Chapter VII Chemotherapy Drug Experiment
First, the antibacterial drug experiment
Experiment 7. In vitro antibacterial activities of1norfloxacin, ofloxacin and ciprofloxacin.
Experiment 7.2 Protective experiment of norfloxacin against infection in mice
Second, the anti-tumor drug experiment
Experiment 7.3 Preliminary Screening of Antitumor Drugs by Methylene Blue Test Tube Method
Experiment 7.4 5-FU's experimental treatment of sarcoma S 180 in mice.
Experiment 7.5 Transplantation of human cancer cells in the renal capsule of mice (nude mice)
Chapter VIII Contraceptive Experiment
Experiment 8. Antiovulation effect of1norethindrone (mating method)
Chapter 9 Anti-aging Drug Experiment
First of all, genetics
Experiment 9. 1 Drosophila life test
Second, the free radical theory
Experiment 9.2 Determination of Lipid Peroxide
Experiment 9.3 Determination of Superoxide Dismutase
Third, the impact on immune function.
Experiment 9.4 Immune Organ Weight Method
Experiment 9.5 Determination of Mononuclear Phagocytosis
Fourth, the influence on stress ability.
Experiment 9.6 Hypoxia Tolerance Experiment in Mice
Experiment 9.7 Cold tolerance experiment of mice
Experiment 9.8 Mouse Swimming Experiment
5. Central nervous system.
Experiment 9.9 Determination of Monoamine Oxidase B Activity
Chapter 10 Application of Cell Culture Technology in Pharmacological Research
Experiment 10. 1 neuron culture
Experiment l0.2 culture of rat brain microvascular endothelial cells
Experiment l0.3 subculture method of primary cells
Experiment/Cryopreservation of KLOC-0/0.4 cells
Experiment 10.5 Pc 12 Cell Resuscitation and Culture
Experiment 10.6 tetrandrine protects cultured rat cortical neurons.
Experiment 10.7 effect of cyclosporine a on rhodamine 123 uptake in rat brain microvascular endothelial cells
Experiment 10.8 effect of nifedipine on Pc induced by high potassium. : the effect of intracellular calcium elevation
Experiment 10.9 Primary culture method of neonatal rat cardiomyocytes
Experiment 10. 10 Primary culture of bovine thoracic aortic endothelial cells
Chapter II XI Drug Safety Evaluation
First, the toxicity test of drugs
Experiment/determination of acute LD50 of kloc-0/1.1procaine.
Experiment 1 1.2 Mutation test of Salmonella typhimurium
Experiment 1 1.3 micronucleus test of mouse bone marrow polychromatic erythrocytes
Second, the preparation of safety limit test
Experiment 1 1.4 pyrogen inspection
Experiment 1 1.5 stimulation test
Experiment 1 1.7 antihypertensive substance test
Experiment 1 1.8 hemolysis test
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