Press: Chemical Industry Press

Release time: 201165438+1October 24th.

ISBN: 9787 122003768

Format: 16

Pricing: 24.00 yuan This book is a textbook for the 11th Five-Year Plan of higher vocational colleges. This textbook not only gives a brief and necessary introduction to the basic theory and knowledge of plant tissue culture, but also focuses on various practical techniques of plant tissue culture, including laboratory equipment and usage methods, basic techniques of plant tissue culture, rapid propagation and virus-free techniques, factory production techniques of plant tissue culture seedlings, and more than 30 common practical techniques of tissue culture of fruit trees, vegetables, medicinal plants, gardens and ornamental plants, taking into account some major types of plant tissue culture in southern China. In order to cultivate students' practical ability, this textbook specially arranges the training content of unit 13, so that students can get systematic training and master practical operation techniques. At the same time, the book also absorbed the latest achievements and advanced experience of plant tissue culture in recent years. Thinking questions are attached at the end of each chapter of the textbook for students to think, review and practice.

This book can be used as teaching materials for biology, landscape architecture and horticulture majors in higher vocational and technical colleges and ordinary undergraduate colleges, as well as teaching materials and reference books for related majors in secondary specialized schools, correspondence courses in junior colleges and adult colleges, as well as training materials for plant tissue culture techniques and self-study books for plant tissue culture enthusiasts and producers. Introduction 1

I. General concept of plant tissue culture 65,438+0

Brief history of plant tissue culture II

Three. Application of plant tissue culture in production 6

Thinking about question 8

Chapter 1 Basic conditions and methods of plant tissue culture

General technology 9

Section 1 Laboratory and Main Equipment 9

I. Design Principles of Plant Tissue Culture Laboratory 9

Second, the composition of plant tissue culture laboratory and its

Function 9

Iii. Sterile operation equipment 1 1

IV. Common Tools 12

V. Common Instruments and Equipment 13

Culture medium in the second quarter 14

I. Types and components of culture medium 14

Second, the preparation of culture medium 16

Three. Method for preparing culture medium 16

Section III Selection and Treatment of Explants 17

I. Type of explants 17

Disinfection of explants 18

Three. Inoculation and culture of explants 19

The fourth quarter aseptic technology 20

I. Disinfection and sterilization technology of test materials and instruments 20

Second, the aseptic operation requirements 2 1

Three. Detection of air pollution in sterile room 2 1

Iv. aseptic operation technology 2 1

Section 5 Environmental Condition Control of Plant Tissue Culture 22

I. Temperature 22

Two. Lighting 22

Three. Humidity 23

Four. Gas 23

Thinking about question 23

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