After nearly two years of hard work, the book Pharmaceutical Cell Biology was finally completed and ready for printing. In the relief, writing group

All staff are looking forward to academic exchanges and communication with readers through this book.

Cell biology is one of the most active biological disciplines, and its knowledge structure is updated quickly, while the pharmaceutical edition of cell biology books is domestic.

There is no precedent for reference. In order to meet the actual needs of discipline development, only some changes have been made in the course of cell biology in domestic pharmaceutical colleges.

You can choose cell biology or medical cell biology textbooks, which deviates from the passive situation of pharmacy major.

Scientists did their best to write this book.

Since this book is mainly a basic biology textbook for pharmaceutical undergraduates, we not only focus on the requirements of the textbook in the process of compiling.

Basic and systematic, but also pay full attention to the novelty of content and the rationality of knowledge structure. The main line of this book

According to the characteristics and trends of the current cross-development of cell biology and pharmacy, from cell, ultrastructure and molecular water

The law and essence of cells in life activities are expounded at different levels, with special emphasis on the closeness of cell biology and pharmacy.

Close contact, and provide a certain length of pharmaceutical examples to help pharmaceutical readers understand cell biology and

Hold on tight This book tries to make readers not only master the basic theory and knowledge of cell biology, but also enhance their understanding and reaction to pharmaceutical knowledge.

Use.

Although this book is written according to actual needs, it is the first attempt after all. Editors deeply feel that their knowledge level and ability are limited.

Inappropriateness, omission and even mistakes are inevitable in the scope of materials and the depth of writing. Please criticize and correct me when reprinting, so as to make efforts.

Mandatory improvement and correction.

compilers

September 2005

About the author: Content: Introduction of Chapter 1 (1)

Executive summary (1)

Section 1 Overview of Cell Biology (1)

I. Research contents of cell biology (1)

Second, a brief history of cell biology development (5)

Three. Cell Biology and Nobel Prize (9)

Cell Biology and Modern Pharmacy (1 1)

1. Cell biology is the basic theory of modern pharmacy (1 1)

Second, the application of cell biology research results and technology in the field of pharmacy (12

)

Iii. Significance of drug cell biology (19)

Thinking about problems (20)

References (20)

The second chapter cell overview (22)

Executive summary (22)

The first section of the basic biological significance of cells (22)

A, the cell is the basic structural unit of biological organisms (22)

Second, cells are the basic unit of metabolism and function of biological organisms (23)

Three, the cell is the basic unit of the growth and development of biological organisms (23)

Four, the cell is the basic unit of heredity (23)

Chemical composition of cells in the second quarter (23)

Section 3 Cell Morphology and Size (24)

I. Cell Morphology (24)

Second, the size of the cell (25)

Three. The unit of measurement of the cell (25)

The fourth quarter prokaryotes and eukaryotes (26)

First, the structural characteristics of prokaryotic cells (26)

Second, the structural characteristics of eukaryotic cells (27)

Three, the comparison of the basic characteristics of prokaryotic cells and eukaryotic cells (29

)

Section 5 Cells and Drug Targets (3 1)

First, the concept of drug target (3 1)

Second, the drug target cells (3 1)

Three. Application Status of Targeted Drugs in Anti-tumor Research (33)

Thinking about problems (33)

References (33)

Chapter III Research Methods and Techniques of Cell Biology (35)

Executive summary (35)

Section 1 Microscopic observation technology of cell morphology (35)

A Brief History of Microscope Development (35)

Second, the classification of the microscope (37)

Three, the basic concept of microscopy and imaging principle (38)

Four, commonly used optical microscope (44)

Verb (abbreviation of verb) Electron microscope (48)

Six, the application of microscopy in the field of pharmacy (58)

Cell chemistry technology in the second quarter (63)

I. Principles and Methods of Enzyme Cytochemistry (64)

Second, the principle and method of immunocytochemistry (65)

Three, autoradiography (67)

Four, in situ hybridization technology (69)

Verb (abbreviation of verb) Problems and prospects (69)

Section III Gradation, Separation and Analysis of Cells and Their Components (70)

I. Isolation and purification of cells (70)

Second, the classification and separation of cell components (73)

Three. Integrated application of cell separation and purification technology (77)

Four, the color analysis of cell components (78)

Verb (abbreviation of verb) Flow Cytometry and Its Application (79)

The fourth quarter cell culture and cell pharmaceutical engineering (85)

I. Overview of Cell Culture (85)

Animal cell culture and Caco-2 cell model (88)

Three, the main technology and development of cell engineering pharmacy (93)

Section 5 Functional Genomics and Its Important Research Techniques (97)

I. Definition and Connotation of Functional Genomics (97)

Second, the important research technology of functional genomics (98)

Think about the problem (10 1)

References (102)

Chapter IV Cell Membrane (103)

Executive summary (103)

Section 1 Chemical Composition and Structural Characteristics of Biofilm (104)

I. Chemical Composition of Biofilm (104)

Second, the molecular structure model of cell membrane (1 10)

Three. Basic characteristics of cell membrane (1 12)

Section II Transmembrane Transport of Substances (1 16)

I. Transmembrane Transport of Small Molecules and Ions (1 17)

2. Membrane Bubble Transport of Macromolecules (124)

Section III Membrane Surface Receptors and Mediated Major Signal Transduction (129

)

Ion channel receptor (13 1)

2.g protein-coupled receptor and its signal transduction (134)

Enzyme-coupled receptor (142)

4. Receptor theory and clinical medication (147)

Section IV Cell Membrane Abnormality and Diseases (148)

1. Abnormal cell membrane transport system (149)

2. Abnormal cell membrane receptor (149)

Iii. Cell membrane and tumor (150)

Four, cell membrane damage (15 1)

Section 5 Research and Application of Cell Membrane in Pharmacy (152

)

I. Interaction between Drugs and Cell Membrane (152)

Second, the hot topic of cell membrane research (158)

Three, cell membrane technology and its application in pharmaceutical research (158

)

Thinking about problems (164)

Reference (164)

Chapter 5 Endometrial System (166)

Executive summary (166)

The first part studies the methodology of endometrial system (167)

I autoradiography (168)

Second, the fluorescent protein technology (168)

Biochemical analysis of subcellular components (168)

Iv. Cell-free system (168)

V. Mutation technology of genetic strains (169)

The second endoplasmic reticulum (169)

1. Basic structural characteristics of endoplasmic reticulum (170)

Chemical composition of endoplasmic reticulum (17 1)

Endoplasmic reticulum type (172)

Fourth, the function of endoplasmic reticulum (174)

Endoplasmic reticulum and diseases (183)

Molecular chaperone of intransitive verbs and its application (185)

Seven. Prospect of endoplasmic reticulum research (188)

Golgi apparatus in the third quarter (188)

I. Basic characteristics of Golgi apparatus (190)

Second, the function of Golgi apparatus (194)

Three, the pathological state of Golgi apparatus (203)

Four, Golgi apparatus and pharmaceutical research promote each other (204)

The fourth quarter lysosomes (205)

I. Basic structural characteristics and classification of lysosomes (205)

Second, the function of lysosomes (207)

Three. Formation of lysosomes (2 10)

Iv. lysosomes and diseases (2 12)

Verb (abbreviation of verb) Lysosome Related Drug Application (2 13)

Section 5 Microsomes and Drug Metabolism (2 17)

1. Microsome and cytochrome P450 enzyme system (2 18)

Second, the basic concepts and methods of drug metabolism research (22 1)

Three. Examples of important CYP oxidative metabolic enzymes (229)

Thinking about problems (234)

References (235)

Chapter VI Mitochondria (237)

Executive summary (237)

The first section of the biological characteristics of mitochondria (237)

I. Morphology and Structure of Mitochondria (238)

II. Chemical Composition of Mitochondria and Localization of Enzymes (240)

Third, the proliferation of mitochondria (242)

Four. Semi-autonomy of Mitochondria (243)

The main function of mitochondria in the second quarter (246)

I. oxidation in eukaryotic cells (247)

Secondly, oxidative phosphorylation is the main link of metabolic energy conversion (249)

In the third quarter, mitochondria and medicine (256)

A pathological process of mitochondrial changes and diagnosis of mitochondrial diseases (256

)

Second, the effects of drugs and poisons on mitochondria (257)

III. Preparation Technology of Mitochondrial Targeted Drugs (262)

Four, mitochondria and diabetes (264)

Verb (abbreviation for verb) Mitochondria and Apoptosis (264)

Thinking about problems (265)

References (265)

Chapter VII Nucleus (267)

Executive summary (267)

Section 1 Ultrastructure and Function of Nuclei (268)

I. Ultrastructure and function of nuclear membrane (268)

Second, the structure of chromatin and chromosome construction (272)

Three, the ultrastructure and function of nucleoli (284)

Four, nuclear matrix (nuclear skeleton) (288)

Five, the function of the nucleus (289)

Section II Diseases Related to Nuclear Abnormality and Their Treatment (29 1)

I hereditary diseases (29 1)

Second, the malignant tumor (294)

Thinking about the problem (2) 294

References (295)

Chapter 8 Ribosomes (296)

Executive summary (296)

Section 1 Morphology, Structure and Existing Types of Ribosomes (297)

I. Morphological structure of ribosomes (297)

Second, the type of ribosome (297)

Physical and chemical properties of ribosomes in the second quarter (298)

The third quarter ribosome self-assembly (299)

The function of the ribosome in the fourth quarter (300)

I. Types of Synthetic Proteins (30 1)

Second, the biosynthesis of protein (302)

Section 5 Changes of Ribosomes under Abnormal Conditions (308)

Section VI Drugs Affecting the Synthesis of protein (308)

I. Regulation of Hemoglobin Synthesis by Heme (309)

Second, the regulation of protein synthesis by interferon (309).

Third, the effect of antibiotics on protein biosynthesis (309)

Thinking about problems (3 10)

References (3 10)

Chapter 9 Cytoskeleton (3 1 1)

Executive summary (3 1 1)

Section 1 Overview of Cytoskeleton (3 1 1)

I. Concept and main functions of cytoskeleton (3 1 1)

Second, the genetic research method of cytoskeleton (3 13)

Section II Microfilaments (3 14)

1. Molecular structure of microfilament (3 14)

Two. Microfilament binding protein (3 16)

Three, muscle contraction system (3 19)

Four, the function of microfilament (322)

Five, a new method to study microfilament genetics (324)

Section III Microtubule (324)

I. Molecular structure of microtubules (324)

Microtubule binding protein 326

Three, microtubule organizing center (327)

Four, the function of microtubules (329)

The fourth intermediate fiber (332)

I. Types of Intermediate Fibers (332)

Second, the molecular structure of intermediate fiber (334)

Three. Intermediate fibronectin (335)

Four, the role of intermediate fiber (335)

Verb (abbreviation of verb) Comparison of Three Cytoskeletons (336)

Section 5 Cytoskeleton Proteins and Diseases and New Drug Development (336)

1. Abnormal expression of cytoskeleton protein and examples of diseases (336

)

Second, the research and development of microtubule inhibitors as anti-tumor drugs (338)

3. Functional genomics provides new opportunities for cytoskeleton research.

(347)

Thinking about problems (348)

References (348)

Chapter 10 Cell Proliferation (350)

Executive summary (350)

Section 1 Basic Concepts of Cell Cycle (35 1)

I. What is the cell cycle (35 1)

Second, the cell synchronization (353)

Mitosis in the second quarter (354)

A, the type of cell division (354)

Second, the basic process of mitosis (354)

In the third quarter meiosis (363)

I. Interval (365)

Second, the split period (365)

The fourth quarter cell cycle regulation (369)

I. Overview of the Research Background of Cell Cycle Regulation (369)

Second, the main regulatory factors of cell cycle and their regulatory methods (374)

Iii. Regulation of DNA Replication (38 1)

Four, the adjustment of cell cycle checkpoint (382)

Verb (abbreviation of verb) Regulation of growth factor (384)

Effect of protein synthesis of intransitive verb on cell proliferation (384)

Section V Functional Genomics Research Examples of Yeast Cell Cycle Regulation (385

)

First, look for genes that are expressed periodically (385)

2. The gene with the highest transcription level in M phase and G 1 phase (386)

3. Gene with the highest transcription level in S phase and G2 phase (386)

Transcriptional Regulation of Periodically Expressed Genes (386)

Verb (abbreviation for verb) Conservation of gene expression regulated by cell cycle (387)

Section VI New Drug Development Based on Cell Cycle Related Mechanism (389

)

First, the application of cell cycle research in the development of new anti-tumor drugs (389)

Second, the application of cell cycle research in the development of antiviral and antifungal drugs (

395)

Three, using cell cycle marker molecules to study the mechanism of drug action and screen new drugs (395)

Thinking about the problem (2) 396

References (397)

Chapter 11 Cell Differentiation (398)

Executive summary (398)

Concept of cell differentiation and potential changes of cell differentiation during embryonic development (398)

A, the concept and characteristics of cell differentiation (399)

Second, the main signs of cell differentiation and research methods (408)

Three. Potential changes of cell differentiation during embryonic development (4 10

)

Section II Molecular Mechanism of Cell Differentiation and Regulation of Gene Expression (4 14)

1. Molecular mechanism of cell differentiation (4 14)

2. Regulation of cell differentiation gene expression (4 15)

Section III Factors Affecting Cell Differentiation (4 19)

First, the influence of cell internal components on cell differentiation (42 1)

Second, the influence of location information on differentiation (422)

Three. Induction and inhibition of cell differentiation by external signals (423

)

Section 4 Application of Cell Differentiation and Related Techniques in Tumor Research (426

)

A, cell differentiation and tumor (426)

Second, the application value of stem cell research and tumor (433)

Three, tumor and induced differentiation (439)

Fourthly, protein omics was used to study the drug targets of tumor-induced differentiation (

442)

Thinking about problems (445)

References (445)

Chapter 12 Apoptosis and Aging (446)

Executive summary (446)

Part I: Characteristics and molecular mechanism of apoptosis (447)

I. Morphological and biochemical characteristics of apoptosis (447)

Second, the difference between apoptosis and necrosis (452)

Three or four stages of apoptosis (453)

Four. Factors affecting apoptosis (459)

Five, apoptosis detection technology (460)

In the second quarter, the application prospect of apoptosis in drug development (463

)

A, abnormal apoptosis and disease (463)

Second, the application prospect of apoptosis drugs (464)

Cell senescence in the third quarter (470)

1. Mechanism of cell senescence (47 1)

Second, anti-aging drugs (476)

Thinking about problems (480)

Reference (480) introduces in detail:

Pharmaceutical cell biology is the first specialty in China that combines cell biology with pharmacy organically, and it is oriented to pharmaceutical colleges and universities all over the country.

Basic biology textbooks for undergraduates of all majors in our school. Based on the theory, principle and technology of cell biology,

To study its application in new drug research and development, pharmaceutical research and drug production. Chapter *** 12 of this book covers pharmaceutical cell biology.

Basic theories and some research hotspots involved in science include introduction, cell overview, research methods, cell membrane and intima.

System, mitochondria, nucleus, ribosome, cytoskeleton, cell proliferation, cell differentiation, cell aging and apoptosis, and in

Relevant pharmaceutical knowledge and applications are integrated into each chapter. I believe that the publication of this book will inspire readers and make them easier to understand.

Understand the relevant knowledge and technology of cell biology and pharmacy.